Transcribed Image Text: A lac operon containing one mutation was cloned into a plasmid, which was introduced by transformation into a bacterium containing a wild-type lac operon. The three genes
Transcribed Image Text: A lac operon containing one mutation was cloned into a plasmid, which was
introduced by transformation into a bacterium containing a wild-type lac
operon. The three genes of the chromosomal operon were rendered nonin-
ducible in the presence of the plasmid.
(a) kind of mutation in the plasmid operon could have this effect?
(b) Suppose the result of transformation was to cause the three plasmid lac
genes to be expressed constitutively, at a high level. type of plasmid
gene mutation could have this result?