Determination of MW by Gel electrophoresis Transcribed Image Text: 2.7 Problem in the Determination of Molecular Welght by Gel Electrophoresis A puri- fied recombinant protein is analyzed for molecular weight

Determination of MW by Gel electrophoresis Transcribed Image Text: 2.7 Problem in the Determination of Molecular Welght by Gel Electrophoresis A puri-
fied recombinant protein is analyzed for molecular weight by SDS-PAGE at pH 8.5. From
the protein sequence deduced from the gene that was expressed in bacteria, the protein is
expected to have a molecular weight of 44,000. However, the molecular weight of the pro-
tein is found by SDS-PAGE to be 52.000. Explain the reason or reasons for this difference
in molecular weight. calculation could you make to explain this discrepancy?
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