Cyanide is an inhibitor of Complex IV of the electron transport chain, i.e., it inhibits the reduction of oxygen to water. Dinitrophenol is a drug that collapses the H +

Cyanide is an inhibitor of Complex IV of the electron transport chain, i.e., it inhibits the reduction of oxygen to water. Dinitrophenol is a drug that collapses the H + gradient across the inner mitochondrial membrane. You are given 2 bottles labeled “A” and “B”.  One of them has cyanide and the other has dinitrophenol.  You are tasked with identifying which of A or B is cyanide and which one is dinitrophenol. You are provided with the following items: A suspension of live, functioning mitochondria A pH-sensitive dye that can permeate both mitochondrial membranes (inner and outer and is red at low pH and blue at high pH. Make a flow-chart to show how you would do an experiment with the items provided to identify which bottle is cyanide and which is dinitrophenol.

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