12 Transcribed Image Text: To select the correct statement about ion exchange chromatography below, consider the pl and the expected net charge of the following two peptides at pH 7.0:
12 Transcribed Image Text: To select the correct statement about ion exchange chromatography below, consider the pl and the
expected net charge of the following two peptides at pH 7.0:
• Peptide X – ETSGD
• Peptide Y – ARKLFK
At pH 7.0 peptides X and Y will both bind equally weakly to either an anion or cation column.
At pH 7.0 peptide X will bind to a cation exchange column more tightly than peptide Y.
At pH 7.0 peptides X and Y will both bind equally tightly to either an anion or cation column.
At pH 7.0 peptide X will bind to an anion exchange column’more tightly than peptide Y.